Education

PhD, Experimental Pathology, Case Western Reserve University, Cleveland, OH. January 1994. Thesis title: “A yeast gene that affects both nuclear export and import.”

BA, University of Delaware, Newark, DE. May 1987. Degree with Distinction. Major: Biology, Minor: History; Junior Year Abroad, 1985-1986, University of Lancaster, Lancaster, UK

Teaching Experience

Assistant Professor, Fall 2008-present, Dept. Biological Sciences, York College of PA, York, PA.

BIO230, Microbiology, lectures and labs, 80 students/semester

Adjunct faculty, Fall 2007, Dept. of Biology, University of Virginia, Charlottesville, VA.

BIOL312, Fundamentals of Microbiology, lecture section, 150 student class.

Adjunct faculty, 8/06-present, Germanna Community College, Locust Grove, VA.

BIO101, General Biology I (2 semesters), lecture and laboratory sections, 20 student classes.

BIO205, Introduction to Microbiology, lecture and laboratory sections, 16 student class.

Research Experience

Assistant Professor of Microbiology, 9/08-present, Dept. Biological Sciences, York College of Pennsylvania

· Research interests: glycosylation in pathogenic fungi

Instructor of Research, 7/03-present, Dept. of Pathology, University of Virginia.

· Research interests: molecular characterization of surface glycoproteins of C. albicans

Research Associate, 9/97- 6/03; Dept. of Pathology, University of Virginia.

· Research interests: regulation of cell surface hydrophobicity in C. albicans

Postdoctoral research, 1/94 - 8/97. Dept. of Cell Biology, University of Virginia.

· Research interests: biochemical characterization of eukaryotic exocytic machinery

Graduate research, 8/87 – 12/93. Dept. of Pathology, Case Western Reserve University.

· Research interests: mechanism of mRNA export from the nucleus of yeast

Professional Development

American Society of Microbiology, Conference on Undergraduate Education, Beverly, MA, May 2008. Four day conference and meeting of educators from a variety of teaching and research environments.

August Teaching Workshop, Teaching Resource Center (UVA), 2007

Topics covered include: “Teaching the First Days of Class”, Developing and Presenting Effective Lecture Courses”, Facilitating Classroom Discussion”, Making It Work for Everyone: How to Teach All Students Effectively”, Making Your Presentations Count”

January Teaching Workshop, Teaching Resource Center (UVa), 2008

Topics covered include: “Clickers: engaging students in learning”, “Will this be on the exam? Science exams as tools for engaging students in the learning process”

Extramural Support

Non-traditional secretion in Candida albicans. Hazen, KC: Principal Investigator; Singleton, DR: Co-investigator. National Institutes for Dental and Craniofacial Research. R01-DE015797, 2005-2008

Honors and Awards

National Research Service Award, NIH-NIDR “Homology of proteins functioning in salivary secretion” F32DE005680, 1996-1997

Neuroendocrinology Training Grant, University of Virginia, 1994-1996

National Science Foundation Graduate Fellowship, 1989

Professional Service

Ad hoc reviewer for FEMS Microbiology Letters, Archives of Microbiology

Professional Affiliations

American Society for Cell Biology

American Society for Microbiology

Publications

Hazen, K.C., D.R. Singleton, and J. Masuoka. 2007. Influence of outer region mannosylphosphorylation on N-glycan formation by Candida albicans: Normal acid-stable N-glycan formation requires acid-labile mannosylphosphate addition. Glycobiology 17: 1052-1060

Singleton, D.R., P.L. Fidel, K.L. Wozniak, and K.C. Hazen. 2005. Contribution of cell surface hydrophobicity protein 1 (Csh1p) to virulence of hydrophobic Candida albicans serotype A cells. FEMS Microbiology Letters 244: 373-377

Singleton, D.R., J. Masuoka, and K.C. Hazen. 2005. Surface hydrophobicity changes of two Candida albicans serotype B mnn4Δ mutants. Eukaryotic Cell 4:639-648

Singleton, D.R.; and K.C. Hazen. 2004. Differential surface localization and temperature-dependent expression of the Candida albicans CSH1 protein. Microbiology. 150:285-292

Wu, J; J. Hicks. C. Ou, D. Singleton, J. Borillo, and Y. Lou. 2001. Glomerulonephritis induced by recombinant Col4α3NC1 is not associated with antibody to GBM: a potential T cell mediated mechanism. J. Immunol. 167:2388-2395.

Singleton, D.R., J. Masuoka, and K. C. Hazen. 2001. Cloning and analysis of a Candida albicans gene that affects cell surface hydrophobicity. J. Bact. 183: 3582-3588

Hubbard C, D. Singleton, M. Rauch, S. Jayasinghe, D. Cafiso, D. Castle. 2000. The secretory carrier membrane protein family: structure and membrane topology. Mol. Biol. Cell. 11:2933-2947

Singleton, D.R., T.T. Wu, and J.D. Castle. 1997. Three mammalian SCAMPs (secretory carrier membrane proteins) are highly related products of distinct genes having similar subcellular distributions. J Cell Sci. 110:2099-107.

Singleton, D.R., S. Chen, M. Hitomi, C. Kumagai, and A. M. Tartakoff. 1995. A yeast gene that bidirectionally affects nucleocytoplasmic transport. J. Cell Sci. 108:265-272

Kadowaki, T., S. Chen, M. Hitomi, E. Jacobs, C. Kumagai, S. Liang, R. Schneiter, D. Singleton, J. Wisniewska, and A.M. Tartakoff. 1994. Isolation and characterization of Saccharomyces cerevisiae mRNA transport defective (mtr) mutants. J. Cell Bio 126:649-659

Hirose, S; R. Mohney, S. Mutka, L. Ravi, D. Singleton, G. Perry, A. Tartakoff, and M. E. Medof. 1992. Derivation and characterization of glycoinositol-phospholipid anchor-defective human K562 cell clones. J. Biol Chem 267:5272-5278

Singh, N; D. Singleton, and A.M. Tartakoff. 1991. Anchoring and degradation of glycolipid-anchored membrane proteins by L929 versus LM-TK^-mouse fibroblasts: Implications for anchor biosynthesis. Mol Cell Bio 11:2362-2374

Tartakoff, A; S. Fatemi, D. Gupta, D. Kaetzel, P. Lemansky, D. Singleton, N. Singh, and E. Tisdale. 1990. Biosynthesis and transport of lipid-anchored membrane proteins. In Molecular and Cell Biology of Membrane Proteins, Glycolipid Anchors of Cell-Surface Proteins, ed. A.J. Turner, pp. 111-128. Ellis Horwood: England

Singleton, D. R. and D. W. Smith. 1988. Improved assay for rhodanese in Thiobacillus spp. Appl Env Micro 54:2866-2867