CHM 336
Exam One
9/20/00
Dr. Foy

Name__________________                                                                                         Signature____________________

Since the Olympics are on, I thought that we could use drug analysis as the scenario.  You are working with the Olympic Committee to develop protocols for blood and urine analysis.  Let's use some round numbers and assume there are approximately 10000 athletes, and samples need to be screened for 175 different drugs.

    1.    You are able to screen for 25 different drugs each procedure, and a single procedure takes 30 minutes from start to
            finish.  You have an unlimited supply of technicians, but only one instrument per procedure, and the technicians can
            only work 8 hours per day.
            a.    How many athletes could be tested each day?
            b.    If you had ten instruments per procedure how many athletes could be tested?
            c.    Consider cost, and the ideal of fairness in the Olympics, what would be the ideal sampling scheme considering
                    the Olympics lasts 14 days.
            d.    Now back to reality, what would be the most realistic scheme if there were only one instrument per procedure?

            Use a spreadsheet to answer the following;
   2.    The analysis of one of the athletes produced the following data for analysis of a growth hormone in one of the athletes.
            a.    If the allowable levels for growth hormone are 10.1ppb +/- 0.1ppb, should this athlete be allowed to compete?
            b.    Report the average, standard deviation, 90% confidence interval, Q-test, and compare the two trials at the
                    95% confidence level.
 
 
 

Trial 1
Trial 2
10.015ug/L
10.005ug/L
10.029
10.049
10.059
9.992
10.056
9.826
9.899
9.947
10.045
10.002
9.947
10.111
9.958
10.058
10.006
9.996
10.007
10.001

   

 
3.    Create a calibration curve using the data below.
       a.    Analyze the following data and determine the concentration of each sample.
       b.    How reliable is the calibration data?
       c.    Which sample would you suggest using the Standard Addition method, and why?

 
 
 
Standard [ ]
Response
Sample 1
Sample 2
0.20
0.0015
0.0099
0.0031
0.40
0.0025
0.0100
0.0029
0.60
0.0048
0.0097
0.0031
0.80
0.0062
0.0098
0.0033
1.00
0.0075
0.0101
0.0032
1.20
0.0094
0.0091
0.0029
1.40
0.0108
0.0092
0.0033

 
 
 
 

4. The Olympic Committee is concerned that you are not using an appropriate scale, and that you haven't considered
    the difference in density. You quickly sketch a diagram of your electronic balance and correct the average weight
    for buoyancy dair = 0.0011g/mL.